Kinetic and Structural Properties of a Robust Bacterial L-Amino Acid Oxidase

Antibacterial Properties of the Mammalian L-Amino Acid Oxidase IL4I1

L-amino acid oxidases (LAAO) are flavoproteins that catalyze the oxidative deamination of L-amino acids to a keto-acid along with the production of H₂O₂ and ammonia. Interleukin 4 induced gene 1 (IL4I1) is a secreted LAAO expressed by macrophages and dendritic cells stimulated by microbial derived products or interferons, which is endowed with immunoregulatory properties. It is the first LAAO d...

The L-amino acid oxidase of Neurospora.

In 1944 one of us described a n-amino acid oxidase in extracts of Neurospora crassa (1). Except for slight activity against L-glutamate, no oxidation of L-amino acids was observed. Recently a means for inducing the formation of a soluble L-amino acid oxidase by the mold was reported by Bender, Krebs, and Hor0wit.z (2). This is accomplished by reducing the biotin content of the basal medium (3) ...

Inactivation of snake venom L-amino acid oxidase by freezing.

The L-amino acid oxidase of Crotalus adamanteus is inactivated by storage in the frozen state at temperatures between -5” and -60°, with maximal inactivation being observed at approximately -20”. The rate of inactivation is dependent on the pH of storage and on the ionic composition of the medium. The inactivation is accompanied by changes in the visible absorption spectrum and by a decrease in...

Crystalline L-amino acid oxidase of Crotalus adamanteus.

Enzymes that catalyze the oxidation of amino acids have been found in microorganisms, mammals, and lower animals. As yet, few of these have been obtained in a state of high purity, and many studies on these enzymes have therefore been carried out with relatively crude preparations. The detailed mechanism of the flavoprotein-cat.alyzed oxidative dcamination of amino acids remains to be <Jlucidat...

Yeast D-Amino Acid Oxidase: Structural Basis of its Catalytic Properties